Western Blot:Article Title: PAPγ associates with PAXT nuclear exosome to control the abundance of PROMPT ncRNAs
Article Snippet: .. Target Reference Supplier Dilution (immunoblot) CPSF6 A301-356A Bethyl Laboratories 1/1000 Flag M2, F1804 Sigma-Aldrich 1/1000 HA SC-7392 SCBT 1/1000 Histone H3 Ab1791 Abcam 1/5000 IgG mouse SC-2025 SCBT 1/5000 IgG rabbit SC-2027 SCBT 1/5000 MTR4 (IP) ab70552 Abcam - MTR4 (WB) A300-614A BethylLaboratories 1/1000 MTR4 (RIP and WB) 12719-2-AP Proteintech 1/1000 PABPN1 66807-1-Ig Proteintech 1/1000 PAPg (WB ) A302-427A BethylLaboratories 1/1000 PAPg (IP, WB, ChIP, ChIP-seq) 24284-1- AP Proteintech 1/1000 PAPg (IP, ChIP) A302-426A BethylLaboratories - RBM26 (IP, WB) A301-215A Bethyl Laboratories 1/1000 RBM27 A301-234A Bethyl Laboratories 1/5000 RNAPII F12; SC-55492 SCBT - Sp1 SC-59 SCBT 1/200 Tubulin DM1A, T6199 Sigma-Aldrich 1/10000 YTHDC1 A305-096A Bethyl Laboratories 1/5000 YTHDC2 A303-025A Bethyl Laboratories 1/5000 ZC3H3 A301-878A Bethyl Laboratories 1/5000 ZC3H14 A303-992A Bethyl Laboratories 1/5000 ZFC3H1 A301-457A BethylLaboratories 1/1000 Primers for RT-qPCR (PROMPTs) Name Forward Reverse EIF4B GGCGAAATGGATGTGCTGAA CGCCTGTAATCCCAGCTACT GDI2 GGTTTCATTGGGAAGCCGATC GCTTCTGCTTACACCTGTGTC KMT2A AAACCTACGTCCCTCCACTG GCGCTGTAACAACGGAATCT MAN1A2 GAGCGCGAAAGAAGAGAAGG AGCCATTAACTCCGGTGACA MRPL20 TCTAGCGTGGGTGACAGATC CATGCACTTGTGGTTCAGCT PPP4R33 CCCATCCCCGCTATATTCGT ACACTGACCCACGTTGTAGT RCOR3 GGAGTGAGTGCTGTGGAGTG ATAAACAGCCCCCAATGCCA RPL8 GCCAAGTGGTTGAGTGACCT CCTGCAAACCATGACGTTCC SLC4A1 ACCTCTCCAACACAAGCTCA CACAGCACTGCGATCTCTTC ZMPSTE24 AGGAGGCTAGGAAGGACTGA CGTCCACTCTCTCATACGCT Primers for ChIP and Re-ChIP Name Forward Reverse EIF4B CTCTTTCCCTCTCCCAACAT CGACTTCAGGAAATCAGTGG GDI2 TCTGTGCCCTCCTTGTTTAG AAACCTGGCTTTCACTTTCA KMT2A CGAGGCCGCTATACAGATTG GTGAAGTGAAGCAGCGAGAG PPP4R3b GTTGGATGGCACCAGTATGT CTTCCTTTCTGTCCCTGGTT RCOR3 CAGAGAGGGCAAAGAGTCC CCCAACCAAGCGCTAATAAA ZMPSTE24 GTTGATGCAACACAGCTCAG CAGACACACTCTTCCCCTTG Table S2. ..
Chromatin Immunoprecipitation:Article Title: PAPγ associates with PAXT nuclear exosome to control the abundance of PROMPT ncRNAs
Article Snippet: .. Target Reference Supplier Dilution (immunoblot) CPSF6 A301-356A Bethyl Laboratories 1/1000 Flag M2, F1804 Sigma-Aldrich 1/1000 HA SC-7392 SCBT 1/1000 Histone H3 Ab1791 Abcam 1/5000 IgG mouse SC-2025 SCBT 1/5000 IgG rabbit SC-2027 SCBT 1/5000 MTR4 (IP) ab70552 Abcam - MTR4 (WB) A300-614A BethylLaboratories 1/1000 MTR4 (RIP and WB) 12719-2-AP Proteintech 1/1000 PABPN1 66807-1-Ig Proteintech 1/1000 PAPg (WB ) A302-427A BethylLaboratories 1/1000 PAPg (IP, WB, ChIP, ChIP-seq) 24284-1- AP Proteintech 1/1000 PAPg (IP, ChIP) A302-426A BethylLaboratories - RBM26 (IP, WB) A301-215A Bethyl Laboratories 1/1000 RBM27 A301-234A Bethyl Laboratories 1/5000 RNAPII F12; SC-55492 SCBT - Sp1 SC-59 SCBT 1/200 Tubulin DM1A, T6199 Sigma-Aldrich 1/10000 YTHDC1 A305-096A Bethyl Laboratories 1/5000 YTHDC2 A303-025A Bethyl Laboratories 1/5000 ZC3H3 A301-878A Bethyl Laboratories 1/5000 ZC3H14 A303-992A Bethyl Laboratories 1/5000 ZFC3H1 A301-457A BethylLaboratories 1/1000 Primers for RT-qPCR (PROMPTs) Name Forward Reverse EIF4B GGCGAAATGGATGTGCTGAA CGCCTGTAATCCCAGCTACT GDI2 GGTTTCATTGGGAAGCCGATC GCTTCTGCTTACACCTGTGTC KMT2A AAACCTACGTCCCTCCACTG GCGCTGTAACAACGGAATCT MAN1A2 GAGCGCGAAAGAAGAGAAGG AGCCATTAACTCCGGTGACA MRPL20 TCTAGCGTGGGTGACAGATC CATGCACTTGTGGTTCAGCT PPP4R33 CCCATCCCCGCTATATTCGT ACACTGACCCACGTTGTAGT RCOR3 GGAGTGAGTGCTGTGGAGTG ATAAACAGCCCCCAATGCCA RPL8 GCCAAGTGGTTGAGTGACCT CCTGCAAACCATGACGTTCC SLC4A1 ACCTCTCCAACACAAGCTCA CACAGCACTGCGATCTCTTC ZMPSTE24 AGGAGGCTAGGAAGGACTGA CGTCCACTCTCTCATACGCT Primers for ChIP and Re-ChIP Name Forward Reverse EIF4B CTCTTTCCCTCTCCCAACAT CGACTTCAGGAAATCAGTGG GDI2 TCTGTGCCCTCCTTGTTTAG AAACCTGGCTTTCACTTTCA KMT2A CGAGGCCGCTATACAGATTG GTGAAGTGAAGCAGCGAGAG PPP4R3b GTTGGATGGCACCAGTATGT CTTCCTTTCTGTCCCTGGTT RCOR3 CAGAGAGGGCAAAGAGTCC CCCAACCAAGCGCTAATAAA ZMPSTE24 GTTGATGCAACACAGCTCAG CAGACACACTCTTCCCCTTG Table S2. ..
Quantitative RT-PCR:Article Title: PAPγ associates with PAXT nuclear exosome to control the abundance of PROMPT ncRNAs
Article Snippet: .. Target Reference Supplier Dilution (immunoblot) CPSF6 A301-356A Bethyl Laboratories 1/1000 Flag M2, F1804 Sigma-Aldrich 1/1000 HA SC-7392 SCBT 1/1000 Histone H3 Ab1791 Abcam 1/5000 IgG mouse SC-2025 SCBT 1/5000 IgG rabbit SC-2027 SCBT 1/5000 MTR4 (IP) ab70552 Abcam - MTR4 (WB) A300-614A BethylLaboratories 1/1000 MTR4 (RIP and WB) 12719-2-AP Proteintech 1/1000 PABPN1 66807-1-Ig Proteintech 1/1000 PAPg (WB ) A302-427A BethylLaboratories 1/1000 PAPg (IP, WB, ChIP, ChIP-seq) 24284-1- AP Proteintech 1/1000 PAPg (IP, ChIP) A302-426A BethylLaboratories - RBM26 (IP, WB) A301-215A Bethyl Laboratories 1/1000 RBM27 A301-234A Bethyl Laboratories 1/5000 RNAPII F12; SC-55492 SCBT - Sp1 SC-59 SCBT 1/200 Tubulin DM1A, T6199 Sigma-Aldrich 1/10000 YTHDC1 A305-096A Bethyl Laboratories 1/5000 YTHDC2 A303-025A Bethyl Laboratories 1/5000 ZC3H3 A301-878A Bethyl Laboratories 1/5000 ZC3H14 A303-992A Bethyl Laboratories 1/5000 ZFC3H1 A301-457A BethylLaboratories 1/1000 Primers for RT-qPCR (PROMPTs) Name Forward Reverse EIF4B GGCGAAATGGATGTGCTGAA CGCCTGTAATCCCAGCTACT GDI2 GGTTTCATTGGGAAGCCGATC GCTTCTGCTTACACCTGTGTC KMT2A AAACCTACGTCCCTCCACTG GCGCTGTAACAACGGAATCT MAN1A2 GAGCGCGAAAGAAGAGAAGG AGCCATTAACTCCGGTGACA MRPL20 TCTAGCGTGGGTGACAGATC CATGCACTTGTGGTTCAGCT PPP4R33 CCCATCCCCGCTATATTCGT ACACTGACCCACGTTGTAGT RCOR3 GGAGTGAGTGCTGTGGAGTG ATAAACAGCCCCCAATGCCA RPL8 GCCAAGTGGTTGAGTGACCT CCTGCAAACCATGACGTTCC SLC4A1 ACCTCTCCAACACAAGCTCA CACAGCACTGCGATCTCTTC ZMPSTE24 AGGAGGCTAGGAAGGACTGA CGTCCACTCTCTCATACGCT Primers for ChIP and Re-ChIP Name Forward Reverse EIF4B CTCTTTCCCTCTCCCAACAT CGACTTCAGGAAATCAGTGG GDI2 TCTGTGCCCTCCTTGTTTAG AAACCTGGCTTTCACTTTCA KMT2A CGAGGCCGCTATACAGATTG GTGAAGTGAAGCAGCGAGAG PPP4R3b GTTGGATGGCACCAGTATGT CTTCCTTTCTGTCCCTGGTT RCOR3 CAGAGAGGGCAAAGAGTCC CCCAACCAAGCGCTAATAAA ZMPSTE24 GTTGATGCAACACAGCTCAG CAGACACACTCTTCCCCTTG Table S2. ..
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